Vero Cell Line Transgene Integrations QC (FISH)

For transgenic cell lines via many methods, the integration site is random and, in most cases, not known. Integration of a transgene in Vero cell line can disrupt an endogenous gene, potentially interfering with interpretation of the phenotype. When the transgene insertion site is unknown, zygosity is determined by expensive quantitative PCR-based approach. These limitations often force investigators to maintain transgenic cell lines in a hemizygous state, which may lead to less than desired expression levels of the transgene and make it less efficient and more costly. Fluorescence in situ hybridization (FISH) is a powerful technique that can be used to visualize transgene integration sites and provide a better understanding of transgene behavior.

With many years of experience and in-depth knowledge, Creative Bioarray can use fluorescence in situ hybridization (FISH) to confirm the integration of transgenes and determine their chromosome band location. We guarantee the speed, quality and cost of our service.

Features of Creative Bioarray's Vero Cell Line Transgene Integrations QC (FISH)

cDNA (2kb~) or genomic DNA fragment on banded chromosomes
Confirm the integration of transgenes
Visualize chromosome band location

Deliverable

Full scope support service-from sample preparation through to the final report
Detailed report of insertion and transgene position
Expert interpretation specific to a new transgenic line

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