Chromosome Aberration Analysis

Creative Bioarray provides probe products, analytical testing services, and fluorescence microscope services that can be used to analyze chromosomal structural variations in animal breeding research. Aiming at the problem of parental chromosome analysis that may be encountered in the field of animal breeding, we will tailor a fast analysis FISH solution for you. Let you experience rapid analysis services from sample processing to data processing. Through adaptively improved experimental programs and the combined application of multiple technologies and equipment, we formulate the most suitable customized solutions for customers' research. Choose our FISH technical service, you will enjoy a one-stop scientific research service.

Chromosome Aberration

Chromosomal aberrations include an abnormal number of chromosomes (aneuploidy) and structural abnormalities of one or more chromosomes. Structural aberrations can be divided into stable and unstable types, the latter including acentric chromosomes, dicentric chromosomes, and circular chromosomes. Unstable aberrations are usually lost with mitosis and cannot become part of the individual's karyotype. Stable aberrations are further divided into balance and imbalance, and whether there is a loss or increase of genetic material is the characteristic of this link. Deletions and duplications are considered unbalanced distortions, while insertions and inversions are balanced distortions, and translocations can be balanced and unbalanced. Translocation is defined as reciprocity, that is, the exchange between two non-homologous chromosomes. In particular, the Robertsonian translocation produces a large chromosome and a small chromosome from the mutual exchange between two heterocentric chromosomes.

Fig 1. A case of using FISH to detect Robertson's translocation (dog). (Szczerbal I et al. , 2021)

Chromosomal Aberration Analysis in Breeding

Generally, balance aberrations have no effect on the phenotype, because neither gene is lost nor gained, and unbalanced aberrations are usually fatal during embryogenesis. However, if one (or more) breakpoints that cause abnormal balance are located inside a gene, that gene will be disrupted or dysregulated, leading to a change in phenotype. This is particularly important for animal production and is related to the success or failure of artificial insemination breeding. Cytogenetic analysis helps visualize copy number variations (CNVs) that are commonly associated with certain phenotypes. FISH testing with BAC probes specific to this region can identify duplicates or doublings. Our FISH testing services provide chromosomal access to analyze phenotype-related CNVs using custom synthetic oligonucleotide probes or BCA probes. At the same time, we also provide services such as karyotype analysis, chromosome location, and chromosome structure variation analysis.

Fig 2. Types of structural variation. (Holečková B et al. , 2012)

The FISH technology service provided by Creative Bioarray can be used for efficient and rapid chromosomal aberration analysis. Chromosomal aberration analysis can help researchers accelerate genetic analysis in animal breeding efforts. Our services are delivered with custom probes to help obtain high-quality FISH profiles. If you are interested in FISH analysis solutions, please contact us for cooperation. We look forward to cooperating with you in the near future.

References

1. Szczerbal I, Switonski M. Clinical Cytogenetics of the Dog: A Review[J]. Animals, 2021, 11(4): 947.
2. Holečková B, Schwarzbacherová V, Galdíková M, et al. Chromosomal Aberrations in Cattle[J]. Genes, 2021, 12(9): 1330.
3. Jennings R L, Griffin D K, O'Connor R E. A new approach for accurate detection of chromosome rearrangements that affect fertility in cattle[J]. Animals, 2020, 10(1): 114.