mRNA FISH Service

Creative Bioarray has a lot of project experience in using labeled oligonucleotide probes for FISH analysis and testing of mRNA. Our RNA FISH experimental platform can successfully provide one-stop analysis and testing services from probe construction to publishable data. For different sample types, we have a unique protocol to provide you with a fast turnaround solution for mRNA FISH. By choosing our service, you can easily obtain experimental reports and date analysis reports that can be used for publication.

Single-molecule RNA FISH

Spatial information is essential for exploring the developmental and tissue-level regulation of gene expression. When using reverse transcription PCR, microarrays, or high-throughput sequencing to measure mRNA levels in the entire tissue, it is often necessary to extract RNA from the entire cell population. Therefore, only the relative measurement value of the mRNA content of the entire cell population is obtained. This leads to the loss of information on the quantity and location of mRNA in a single cell. In contrast, single-molecule fluorescence in situ hybridization (smFISH) retains the spatial information of cellular mRNA content and has subcellular resolution across tissues. In situ hybridization (ISH) allows qualitative mRNA detection in intact tissues. However, with the development of single-molecule fluorescence in situ hybridization (smFISH) and subsequent optimization, single-mRNA analysis has been allowed. The benefit is that there is a rapid increase in interest in detecting and quantifying all aspects of mRNA biology in a single cell with a single mRNA sensitivity.

Fig 1. Single Molecule RNA-FISH Hybridization Scheme. (Xie F, et al. 2018)

Customized mRNA FISH Services

Single-molecule fluorescence in situ hybridization (smFISH) can be used for any organism, cell culture, and tissue sectioning. Based on smFISH technology, we provide FISH analysis services that can be used for mRNA of any organism, cell culture and tissue section. The service process includes customized service plan, probe preparation, sample preparation, FISH hybridization and washing microscope service and data service. It should be noted that our mRNA FISH analysis service has a special protocol for each sample type, and we develop the most suitable solution for our customers. We use labeled oligonucleotide probes (probe synthesis for 15-20 nt target-specific sequences) for analysis. This can ensure the specificity of the probe and prevent non-specific probe hybridization. In addition, we also use other quality control steps to reduce background, such as avoiding the disadvantage of high background signal caused by the use of long probes (more than 40nt) and relatively low temperature (20 ℃ below annealing temperature) for hybridization and washing.

Fig 2. Flow chart of mRNA FISH analysis service.

Our Features

• High sensitivity and specificity. Unique probe design provides excellent background noise control;
• Quantify RNA expression. Single cell quantification of RNA expression level;
• Morphological context. Cell-specific expression information within the complete tissue structure;
• Universal application. Compatible with laboratory workflow and clinical sample types;

Creative Bioarray provides FISH technical services from the synthesis of labeled oligonucleotide probes to sample mRNA analysis, helping our customers save costs. You will benefit from our technical expertise and solutions, and work with you to find the most satisfactory scientific research results.

If you are interested in our mRNA FISH analysis service, please contact us for cooperation. We look forward to cooperating with you in the near future.

References

1. Xie F, Timme K A, Wood J R. Using single molecule mRNA fluorescent in situ hybridization (RNA-FISH) to quantify mRNAs in individual murine oocytes and embryos[J]. Scientific reports, 2018, 8(1): 1-11.
2. Trcek T, Lionnet T, Shroff H, et al. mRNA quantification using single-molecule FISH in Drosophila embryos[J]. Nature protocols, 2017, 12(7): 1326-1348.