Humanized Animal Models

The humanized animal is defined as an animal xenotransplant with human cells or cells expressing human gene products. Laboratory animal shares most of their protein-coding genes with humans, but not all aspects of animal biology reflect human biology. As drug development for cancer and autoimmune diseases continues, a growing demand for specialized animal models with human immune systems is growing. And with almost 90% of novel drugs that pass preclinical tests failing in human clinical trials, new approaches to improve the odds are desperately sought. Humanized animal models are beneficial tools for immune-oncology and infectious disease research. They have improved the ability to predict human responses to new therapeutics in clinical development.

Currently, humanized animal models are essential tools for biomedical research and have become increasingly popular over the years. They can be used in various studies, including immunology, cancer research, infectious diseases, and drug development. Thus, the Humanized Animal Model Analysis is also an important part. With years of experience in this field, Creative Bioarray is capable of offering Humanized Animal Model Analysis Services in the most high-quality and cost-effective way.

Humanized Animal Model Tests Available:

Humanized animals that require characterization of species-specific cell types. We can use the RNA FISH method to do this analysis. Accurate and reproducible detection of biomarkers is critical for understanding their role in normal and diseased states. RNA is an excellent indicator of the dynamic genetic expression changes in a cell, but traditional methods that analyze RNA in bulk tissues or cell populations, such as RT-PCR, mask the cell-to-cell variations in gene expression and result in the loss of information on the spatial relationship of the analyzed cells. Mapping RNA expression to single cells can detect virtually any type of tissue derived from various animal models and is particularly valuable when specific, reliable antibodies are not available.

Apart from that, some kinds of humanized animals require characterization of the transgene. For transgenic animals, the integration site is random and, in most cases, not known. In addition, knowledge of where the transgene is integrated is essential for planning of crosses between animals carrying a conditional allele and a given Cre allele in case the alleles are on the same chromosome. When the transgene insertion site is unknown, zygosity is determined by an expensive quantitative PCR-based approach. These limitations often force investigators to maintain transgenic models or cell lines in a hemizygous state, which may lead to less than desired expression levels of the transgene and make it less efficient and more costly. Targeted Integration QC (FISH) and Targeted Integration QC (Locus Amplification & Sequencing) is a powerful technique that can be used to know transgene integration sites and provide a better understanding of transgene behavior.

Quotation and ordering

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References

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5. Deruaz M, Luster AD. BLT humanized mice as model to study HIV vaginal transmission. J Infect Dis 2013; 208(Suppl. 2): S131– 6.