FISH Services on Tissue Microarray

Tissue microarray (TMA) technology provides a high-throughput method for evaluating potential biomarkers and therapeutic targets in archival pathology specimens. The arrangement of multiple cores on a single slide of the tissue array helps to quickly evaluate the molecular changes of hundreds of different tumors on a single slide. TMA sections can be used for any in situ tissue analysis, including fluorescence in situ hybridization (FISH). FISH is a molecular technique that can detect the number and structure of metaphase chromosomes and interphase cell nuclei. TMAs are a powerful tool for the rapid identification of tumor types associated with specific molecular alterations. FISH is commonly used as a prognostic and diagnostic tool for detecting translocations and assessing gene deletions and amplifications in tumors. Performing FISH on TMA allows researchers to determine the clinical significance of specific genetic changes in hundreds of highly characterized tumors. Performing FISH on archival paraffin embedded TMAs is technically possible and can provide key information regarding the diagnostic, prognostic and predictive value of novel biomarkers.

Fig 1. Applications of FISH on TMAs. (Brown L, et al. 2007)

FISH Analysis Services for Tissue Arrays

This service can perform FISH analysis on paraffin-embedded tissue arrays to detect chromosome copy number changes, amplifications, deletions, and rearrangements. One of the determinants of the high-throughput characteristics of tissue arrays is the number of cores in the slices. We currently accept analysis of tissue microarrays with 8-24 cores due to the current observable field of view in tissue slices. The basic process of this service includes order submission, tissue microarray mailing, FISH probe selection, FISH hybridization, imaging and data analysis. In the order submission stage, the customer needs to submit the basic information of the organization array, which is convenient for our experimenters to adjust the plan. Our microarray platform is capable of multicolor FISH analysis. Multi-color FISH analysis can be performed for low-core tissue arrays. At the same time, we provide CISH analysis services for tissue arrays. For details, please refer to CISH analysis services. In addition, this service allows large-scale analysis of single target genes via FISH on multi-core tissue arrays.

Applications of FISH on TMAs

• Detection of Gene Amplifications
• Identification of Fusion Genes
• Detection of Translocations
• Detection of Rearrange
• DNA Copy Number Analysis
• Cancer Related FISH Analysis

Fig 2. Our characteristic organization array FISH analysis service.

Creative Bioarray provides FISH analysis technology services for tissue arrays. The feature of this service is the use of tissue arrays for uniform target analysis. At the same time, we also provide CISH analysis services based on tissue arrays. This service aims to help customers efficiently realize the analysis of chromosome structural variation, translocation, and rearrangement on the tissue array. You will benefit from our technical expertise and internally optimized special microarray FISH analysis protocol. We are confident to provide you with cost-effective and time-effective FISH solutions. If you need chromosome variation analysis or specific gene mapping analysis on organized arrays, please choose our tissue array FISH analysis. If you are interested, please contact us for cooperation. We look forward to cooperating with you in the near future.

References

1. Brown L A, Huntsman D. Fluorescent in situ hybridization on tissue microarrays: challenges and solutions[J]. Journal of molecular histology, 2007, 38(2): 151-157.
2. Kallioniemi A. DNA copy number analysis on tissue microarrays[M]//Tissue Microarrays. Humana Press, Totowa, NJ, 2010: 127-134.