Alien Gene Introgression

Creative Bioarray provides probe design and customization services for crop genome analysis. In view of the current lack of commercialized probes for chromosome analysis and fragment identification in the agricultural field, our probe design service can help you improve your analysis capabilities to a certain extent. For genome visualization problems that may be encountered in the field of ploidy hybrid breeding, we tailor-made project solutions for you, allowing you to experience the whole process service from sample processing to data processing. Through adaptively improved experimental protocols and the combined application of various techniques and equipment, our services can be applied to a variety of sample types and disciplines. Our diverse products and customized solutions for customer projects are our strengths and characteristics.

Alien Gene Introgression

Alien gene introgression lines are mainly produced by crossing crops with their wild source species, such as Brassicaceae and Gramineae. The purpose of the wild relatives produced by this interspecific hybridization is to screen new varieties with ideal agronomic traits, such as disease resistance and stress resistance. Improved interspecific hybridization technology has to a certain extent alleviated the development of crops that are hindered by linkage drag and the low success rate of distant hybridization. Embryo rescue and induction of homologous chromosome recombination are used to achieve successful distant hybridization and useful gene recombination. Molecular markers developed from the genome-wide sequence information of known species can become a source of adequately anchoring foreign chromosome fragments in the introgression line of foreign chromosomes. Integrating appropriate molecular markers into breeding programs for marker-assisted backcrossing can greatly assist in the selection of harmful genes and can facilitate the tracking of the process.

Fig 1. An analysis case of gramineous crops. (Ali N et al., 2016)

FISH and Gene Mapping

The agricultural traits of existing crops can be significantly improved by integrating new genes from different gene pools, especially from wild relatives, into existing crop species. Despite the importance of increasing the genetic diversity of domesticated crops, most foreign genes cannot be fully utilized due to the limitation of deleterious linkage resistance introduced by foreign chromosome segments. Linkage resistance may also be due to the inability of the introduced foreign chromosomal segment to adequately compensate for the loss of its cognate counterpart or too incompatible gene interactions between the foreign donor and recipient lines. In agricultural breeding research programs, researchers can use FISH/GISH techniques to locate foreign genes or incorporated fragments in the offspring of hybrids. These jobs depend on suitable probes, and our probe customization service can help researchers solve probe customization work. Our experts have extensive expertise, please contact us for a solution. We will provide one-stop scientific research services, and you will harvest high-quality chromosome maps.

Creative Bioarray provides a comprehensive FISH solution for the analysis of introgressed alien genes, helping to quickly identify introgressed genes in the research work of crossing-breeding. Our solution will be adjusted according to your research purpose and sample type. You will benefit from our technical expertise and advanced equipment platform. If you have a need for genetic analysis of the alien genes introgression of hybrid offspring, please contact us for cooperation. We look forward to cooperating with you in the near future.

References

1. Ali N, Heslop-Harrison J S P, Ahmad H, et al. Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance[J]. Heredity, 2016, 117(2): 114-123.
2. Dong Z, Ma C, Tian X, et al. Genome-wide impacts of alien chromatin introgression on wheat gene transcriptions[J]. Scientific reports, 2020, 10(1): 1-12.