Naked DNA Biodistribution

Naked DNA Biodistribution (RNA ISH assay) Services from Creative Bioarray provide an unparalleled sensitive and specific method for the safety and efficiency of gene therapy vector and transgene expression analysis in any tissue.

Increases in efficiency have made naked DNA gene transfer a viable method for gene therapy. RNA ISH assay has several advantages in the application of naked DNA biodistribution gene therapy studies which include:

• Localization: RNA ISH assay allows for the localization of specific mRNA molecules and the plasmid DNA at both the cellular and subcellular levels within a tissue context. This can help in determining the exact location where the delivered gene is being expressed.
• Quantification: RNA ISH assay allows for the quantification of the transcript level of genes of interest. This can facilitate the measurement of gene expression levels after the delivery of naked DNA.
• Visualization: RNA ISH assay produces highly specific and bright signals which can be spatially resolved. This can help in visualizing the biodistribution of the delivered gene within the treated individual or model organism.
• Heterogeneity: RNA ISH assay can detect cellular heterogeneity in gene expression within a population of cells. This can be useful in assessing the efficiency and specificity of naked DNA delivery techniques.
• Temporal information: RNA ISH assay can capture dynamic changes in gene expression levels over time providing a temporal resolution that could be highly valuable in the context of gene therapy studies.

Figure 1. DNA vaccines induce adaptive immune responses.

RNA ISH assay provides the detection of the expression of the transferred genes through RNA ISH indirectly provides information about the distribution and the efficacy of the naked DNA-based gene transfer.

Figure 2. RNA ISH probe design strategy.

The transfer of naked DNA for gene therapy is gaining increasing attention for various gene therapy applications and has advantages in terms of ease of DNA preparation, simplicity of use, and lack of immunogenicity. Given its potential in successfully delivering therapeutic genes into targeted cells and tissues with little adverse effects, naked DNA holds the promise of treating a wide range of diseases, from cancer and genetic diseases to infectious diseases. Furthermore, with continuous research and development in delivery techniques and DNA formulation, the efficacy and specificity of Naked DNA gene therapy are expected to be significantly improved in the future, making it a growing field with great potential in medicine. Our Naked DNA Biodistribution services can help you accelerate your gene therapy development.

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References

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