MDCK cells are used in studies of viral infections, and more recently in vaccine production. However, MDCK cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.
Karyotype analysis (G-Banded analysis) can indicate that MDCK cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tool for the detection of instability in cell line development processes. MDCK cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established MDCK Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.
Figure 1. Dog cell karyotype generated from bovine cultured cells. Spread #13 of DSC p4 (left panel) with
its corresponding karyotype (right panel). Chromosome complement: 78,XX
Creative Bioarray's MDCK Cell Chromosomal Aberration Analysis has the following features:
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