Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis

Recombinant adenoviruses are currently the most widely used vectors in human gene therapy trials. However, one essential consideration when evaluating the therapeutic potential and safety profile of an adenovirus vector is its distribution and persistence in various organs and tissues. While qPCR-based methods provide average values for copy number, the RNA in situ hybridization (ISH) technology provides morphology-based, cell-specific quantification of vector DNA, transgene mRNA, and specific biomarkers.

Adenovirus Biodistribution detection is often required for regulatory approval of gene therapy or vaccine products. The US Food and Drug Administration (FDA) and other regulatory agencies require extensive preclinical and clinical testing to ensure the safety and efficacy of these products.

In situ hybridization is a powerful technique used in molecular biology to detect and localize specific nucleic acid sequences within tissue or cell samples. RNA ISH technology can be used to detect adenovirus vector DNA and therapeutic transgene mRNA expression within intact tissue morphology, addressing critical questions on tissue biodistribution, persistence, cellular tropism, and vector promoter activity.  As a leading technology provider, Creative Bioarray's elite scientists have broad experience in Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis (RNA ISH method), which can supply the best service for you.

Features of Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis (RNA ISH method)

(1) Detection of single-copy DNA vectors
(2) Codon-optimized transgene and biomarker expression analysis
(3) Custom probes designed within 1-2 weeks
(4) Fastest turnaround time

Benefits of Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis (RNA ISH method)

(1) Simultaneously visualize in vivo delivery of vector DNA and therapeutic transgene mRNA
(2) Identify cell tropism of Adenovirus vector by multiplexing with cell-type markers
(3) Distinguish transgene from endogenous sequences, at the single nucleotide level
(4) Quantify Adenovirus+ cell number and track persistence over time

Creative Bioarray offers Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis for you as follows:

• Probe design
• Probe synthesis
• ISH staining
• Imaging
• Data analysis

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for considering Creative Bioarray as your Adenovirus Vector/Transgene/Biomarker RNA Biodistribution Analysis partner.

References

1. Hastie E and Samulski RJ. Adeno-associated virus at 50: A golden anniversary of discovery, research, and gene therapy success — a personal perspective. Hum Gene Ther. 2015; 26:257–265.
2. Wang F, et al. RNAscope®: A novel in situ RNA analysis platform for formalin-fixed, paraffinembedded tissues. J Mol Diagn. 2012; 14(1):22–9.
3. Polinski NK, et al. Impact of age and vector construct on striatal and nigral transgene expression. Mol Ther Methods Clin Dev. 2016; 3, 16082.
4. Keeler AM, et al. Cellular analysis of silencing the Huntington's Disease gene using AAV9 mediated delivery of artificial microRNA into the striatum of Q140/Q140 mice. J Huntingtons Dis. 2016; 5(3):239–248.
5. Borel F, et al. Therapeutic rAAVrh10 mediated SOD1 silencing in adult SOD1G93A mice and nonhuman primates. Hum Gene Ther. 2016; 27(1):19–31.