Creative Bioarray provides comprehensive FISH analysis solutions for gene expression analysis and staining in rats for different research purposes, such as comparative gene mapping, chromosomal structure studies, genome evolution, chromosomal aberrations, and characterization of transgene insertions. The high-quality service team and rich professional knowledge give us the confidence to provide customers with high-quality testing services.
Rats are the second most commonly used model organism due to their relatively short lifespan. FISH is an accurate and sensitive cytological technique for visualizing specific DNA sequences on chromosomal preparations. FISH has facilitated the analysis of the rat genome, especially in transgenic techniques to more accurately characterize the assignment of chromosomal locations of inserted genes in rats. As a physical mapping technique, FISH can be used to map clones on chromosomes in metaphase. And because of the correlation between the physical proximity of interphase DNA sequences and their genomic distance, FISH can also be used to locate clones on interphase nuclei. At the same time, hybridization on stretched DNA fibers allows sequencing of clones at significantly higher resolution and also allows estimation of the size of gaps and overlaps in the contig map. Our technical service platform is used to help researchers address projects that apply FISH to different research purposes in rats, from comparative genetic mapping to the study of chromosome structure and genome evolution, chromosomal aberrations, and the characterization of transgene insertions. Our technical services include the development of a full-scale FISH procedure, including the preparation of mitotic chromosomes and DNA fibers from short-term cell cultures for improved resolution.
Fig 1. FISH on rat chromosomes and DNA fibers. (Andrew Jefferson, et al. 2010)
As a widely used laboratory model animal, rats can be used to construct animal models of diseases, analyze gene expression, and so on. Our FISH technology service platform offers several types of FISH technology service options including metaphase FISH and Fiber-FISH for metaphase chromosomes or chromatin fibers, and ZOO-FISH analysis. Our FISH technical services are based on a wide range of custom probes and some commercial probes, and the in-house optimized experimental protocol can be adapted to most types of commercially available FISH probes. Featured FISH technical services performed on rat samples, including techniques for the preparation of mitotic chromosomes and DNA fibers from short-term fibroblast cultures and splenocytes, are designed to provide sufficient technical detail and experimental versatility.
The application of molecular cytogenetics in rats in the field of genetic research is particularly suitable for the study of strategies for the isolation and identification of human disease genes. Our FISH technology services can be used for some of the reported applications of FISH in rat genomes and post-genome studies. Especially in the post-genomic era, FISH is widely used to resolve differences in cellular localization and as a validation technique for copy number variation (CNV).
Fig 2. FISH applications in laboratory rat genomic studies.
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