Adenovirus Vector/Transgene/Biomarker Quantification and Statistical Analysis

Adenoviruses have been used clinically for gene supplementation, vaccination, and as oncolytic virotherapy. To date, there have been over 100 adenovirus type described.  Determining the adenovirus vector distribution is central for the safety assessment of proposed early-phase clinical trials. The RNA ISH assay enables single molecule detection of adenovirus vector DNA, transgene mRNA, and/or specific biomarkers in the tissue at the single-cell level, thus allowing for a high spatial resolution at both the cellular and subcellular levels. Cell type-specific promoter activity, transgene, and cell-specific biomarker expression heterogeneity can be assessed with the RNA ISH assays, as can screening of vector delivery systems for cellular uptake efficiency and extracellular trapping of the vector. Apart from that, RNA ISH can be used for quantitative analysis of adenovirus distribution, allowing researchers to determine the number of infected cells and the amount of viral load within tissues. RNA ISH assay from Creative Bioarray allows visualization and quantification of adenovirus vector presence, transgene, and biomarker expression in treated tissue samples. Following the current FDA guidelines, our experienced scientists will design the most appropriate experiment plan to meet every client's specific requirement.

Benefits of Adenovirus Vector/Transgene/Biomarker Quantification and Statistical Analysis (RNA ISH method)

(1) Adenovirus vector DNA detection and quantification in intact fixed tissue
(2) Quantifiable assays to measure percentage of cells positive for vector, transgene and biomarker expression
(3) Quantify adenovirus + cell number and track persistence over time

Quotation and ordering

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References

1. Hastie E and Samulski RJ. Adeno-associated virus at 50: A golden anniversary of discovery,research, and gene therapy success — a personal perspective. Hum Gene Ther. 2015; 26:257–265.
2. Wang F, et al. RNAscope®: A novel in situ RNA analysis platform for formalin-fixed, paraffinembedded tissues. J Mol Diagn. 2012; 14(1):22–9.
3. Polinski NK, et al. Impact of age and vector construct on striatal and nigral transgene expression. Mol Ther Methods Clin Dev. 2016; 3, 16082.
4. Keeler AM, et al. Cellular analysis of silencing the Huntington's Disease gene using AAV9 mediated delivery of artificial microRNA into the striatum of Q140/Q140 mice. J Huntingtons Dis. 2016; 5(3):239–248.
5. Borel F, et al. Therapeutic rAAVrh10 mediated SOD1 silencing in adult SOD1G93A mice and nonhuman primates. Hum Gene Ther. 2016; 27(1):19–31.