MDBK cell line is a continuous cell line used to propagate virus for vaccine manufacturing. However, MDBK cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.
Karyotype analysis (G-Banded analysis) can indicate that MDBK cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tool for the detection of instability in cell line development processes. MDBK cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established MDBK Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.
Figure 1. Bovine cell karyotype generated from bovine cultured cells. Spread #13 of BSC p28 (lower panel) with its corresponding karyotype (upper panel). Chromosome complement: 60,XX
Creative Bioarray's MDBK Cell Chromosomal Aberration Analysis has the following features:
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