Genomic in situ Hybridization (GISH)

Creative Bioarray provides probe products, analytical testing services, and fluorescence microscope services that can be used for crop genome analysis. Our genomic in situ hybridization (GISH) technology service uses traditional and improved experimental protocols, as well as the combined application of a variety of technologies and equipment so that our services can be applied to a variety of sample types and subject areas. Our diversified products and customized solutions for customers' projects are our strengths and characteristics. Choose our FISH technical service, you will enjoy a one-stop scientific research service.

GISH and Plant Chromosome Analysis

The GISH method is an improved variant of fluorescence in situ hybridization (FISH) and has been widely used in the genome analysis of various plant researches such as hybrid breeding and species evolution. The upgrading of this technology has made it one of the most important technologies in molecular cytogenetics. GISH is a molecular technique with broad application prospects that allows the differentiation of genomes in cells. Using this technique, the genomes in hybrids can be distinguished. Therefore, this method has been applied to hybrid pedigree studies, genetic improvement projects, and polyploid evolution studies. Generally, GISH is used to analyze the meiotic behavior of hybrids and polyploidies to provide information about the relationship between species. GISH technology can help decipher the genome composition of allopolyploid plants. Our service uses nuclear DNA from the genome as a template for probe design, using different nucleotide markers to distinguish them.

Fig 1. Main steps of the genomic in situ hybridization (GISH). (Brammer S P, et al. 2013)

GISH Assay

The development of GISH technology is also based on customized probe sets, and its core is the design and customization of characteristic probe sets. GISH assay is used in the field of plant chromosome analysis and agricultural breeding, and can realize the analysis of genomic polyploid hybrids, partial aneuploidy and recombinant inbred lines. This technique can effectively analyze the karyotypic stability of plant material and indicate the optimal genotype, thereby providing knowledge for assisted selection at different stages of crop improvement.

• Confirm the origin of polyploid chromosomes (distinguish the relationship between polyploid genomes)
• Recognize mosaic chromosomes (foreign chromosomal fragments and chromatin and chromosomal rearrangements)
• The differentiation of parental genomes from different species after somatic cell hybridization
• Observe the genome composition of the entire chromosome or recombination fragment stage
• Detection and differentiation of parental genome in the process of hybrid meiotic chromosome pairing
• The differentiation of different genomes and the identification of chromosomal loci in the offspring of gene introgression breeding (discrimination of metastatic traits)

Creative Bioarray's custom probe service can help customers with GISH analysis needs to design and synthesize probe sets. We have many years of experience in FISH technical services and a mature probe design service system. Probe design outsourcing can help our customers save time and cost. You will benefit from our technical expertise and equipment platform to provide you with the best solution for your needs. If you have relevant research needs or are interested in our FISH service, please contact us for cooperation. We look forward to cooperating with you in the near future.

References

1. Brammer S P, Vasconcelos S, Poersch L B, et al. Genomic in situ hybridization in Triticeae: A methodological approach[J]. Plant Breeding from Laboratories to, 2013: 1-22.
2. Silva G S, Souza M M. Genomic in situ hybridization in plants[J]. Genet Mol Res, 2013, 12(3): 2953-2965.