BHK-21 cell line is a continuous cell line used to propagate foot-and-mouth disease (FMD) virus for vaccine manufacturing. However, BHK-21 cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.
Karyotype analysis (G-Banded analysis) can indicate that BHK-21 cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tool for the detection of instability in cell line development processes. BHK-21 cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established BHK-21 Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.
Figure 1. BHK-21 Karyotype generated from BHK-21 cultured cells. Spread #10 of (left half) with its corres ponding karyotype (right half). Chromosome complement: 20 chromosomes (mainline karyotype plus extra copy of Chromosome 8).
Creative Bioarray's BHK-21 Cell Chromosomal Aberration Analysis has the following features:
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