Since the birth of FISH technology and the improvement of the drawbacks caused by radiation in situ hybridization, it has been undergoing technological innovation. Efforts to use FISH for mid-term high-resolution analysis and automatic karyotype generation have led to the emergence of many different methods. In recent years, various FISH-based multicolor technologies have been developed. The most commonly used multicolor karyotyping techniques include multiple FISH (M-FISH) and spectral karyotyping (SKY), which are due to the technology behind the way that fluorescent dye information is generated. Later, some unique labeling methods and color-changing karyotype analysis were added to the FISH-based karyotype analysis. So far, these techniques have been supplemented by a variety of additional methods. Some of these applications are aimed at improving the resolution of aberrations within chromosomes, while others are dedicated to detailed analysis of specific subregions within the genome. Some of these applications are aimed at improving the resolution of aberrations within chromosomes, while others are dedicated to detailed analysis of specific subregions within the genome. Multicolor FISH (m-fish) refers to the simultaneous use of at least three different ligands or fluorescent dyes to specifically label DNA-excluding counterstains.
Fig 1. 25-color FISH karyogram of normal female metaphase. (Liehr T, et al. 2004)
The development of multicolor FISH (m-fish) has produced many nouns, introducing different names for more or less the same probe set: M-FISH (Multiplex-FISH); SKY (spectral karyotyping); COBRA-FISH (combined binary ratio marker-FISH); or 24-color FISH. Our special m-fish service uses some special multicolor probe sets for chromosome analysis.
|Diversified Probe Selection
m-fish is defined as the simultaneous use of at least three different ligands or fluorescent dyes to specifically label DNA-excluding counterstains. The labeling method usually uses four to seven fluorescent dyes for combination labeling and/or ratio labeling. Combination mark refers to a combination of up to 3 fluorescent dyes, and each fluorescent dye combination is used only once. Ratio labeling refers to the combination and mixing of up to three different ratios of fluorescent dyes per chromosome.
|Our m-fish service provides the following 2 optional services:
|Whole Chromosome M-FISH Services
|The whole chromosome staining m-fish probe sets have been successfully used for the confirmation, improvement and/or characterization of translocations. At the same time, this method can be used to characterize and label chromosomes in clinical genetics, tumor cytogenetics, mutagenesis, radiobiology, mammalian evolution, or mysterious rearrangements of interphase structures. Our full chromosome M-FISH service uses a full chromosome library to dye each chromosome of all chromosomes of different biological samples into another color at the same time. Finally, provide customers with a complete chromosome map of the entire biological sample.
|Specific Chromosome M-FISH Services
|For some customers who have target chromosome analysis, we provide M-FISH service for analyzing the chromosome corresponding to a specific disease or one or more chromosomes of interest. For special problems, customers can choose to add some more probes to the preset probe set, such as single-copy probes (subtelomeric probes), chromosome region-specific probes (acrocentric chromosomes' short-arm probes). In addition, this service also provides related analysis for heterochromatin chromosome probe sets (1q12, 9q12, 16q11.2, 19p1, 19q12, YQ12).
Fig 2. Multicolor technology and FISH characteristic service.
Creative Bioarray provides a variety of optional multiple probe sets for multi-color FISH technical services. You will benefit from our technology platform and unique design to provide you with the best solution. If you are interested in our m-fish service, please contact us for cooperation. We look forward to cooperating with you in the near future.