In situ RNA detection is becoming more and more important for analyzing gene expression in and between intact cells in tissues, and in situ RNA detection techniques (ISH and FISH) have existed for many years. Since its launch, RNA ISH analysis has become a valuable method for locating and quantifying specific nucleic acid sequences in individual cells and tissues, providing information on tissue specificity, cell specificity, and subcellular gene expression at different developmental stages in biology, as well as normal tissues And cells in the diseased tissue. With the development of technology, new branch technologies and technology integration are gradually produced. The FISH technique, which uses fluorescently labeled oligonucleotide probes to hybridize with cellular RNA in situ, has become a powerful technique for quantifying the copy number of a single transcript and the spatial distribution of these molecules in a single cell. In this method, the cells are fixed and permeabilized, and a small amount of fluorescently labeled oligonucleotide probes are introduced into the cells, the sequence of which is complementary to different regions of the RNA of interest. The base pairing between these probes and their target RNA facilitates the specific binding of these probes to their targets, and each target uses multiple probes to concentrate many probes in a small volume of cells occupied by each copy of the RNA Inside, it produces bright fluorescent spots, which can be distinguished from cell background and stray probes. These points can then be counted to determine the number of copies of the target RNA and its location in individual cells and intact tissues.
Fig 1. Schematic representation of the technical development of fluorescent in situ hybridization (FISH). (Young A P, et al. 2020)
As one of the world's leading FISH technology service companies, we have long been committed to the development of RNA-FISH services. The FISH technology service with RNA as the target can realize RNA in situ discovery, functional research, bioinformatics analysis, etc. The molecular targets of our RNA-FISH technology services include coding RNA (mRNA) and non-coding RNA (including lncRNA, siRNA, miRNA, cricRNA). We use our comprehensive knowledge to provide our customers with the most powerful RNA research solutions and extensive expertise to promote the progress of your projects at the most competitive cost. We provide industry-leading FISH analysis and testing services. This customized service that analyzes targets or areas that customers are interested in can accurately meet the direct needs of customers and maximize their benefits. In addition to some traditional FISH methods, our RNA-FISH service also provides analysis of some related technologies, as well as technical services for many FISH variants. Use these services to locate, qualitatively and quantitatively analyze and locate molecules of interest in cell and tissue samples.
Fig 2. The key element of RNA-FISH service.
Optional semi-custom and full-custom solutions;
Various types of customized probes and pre-made probe product selections, such as oligonucleotide probes, PNA probes, LNA probes, etc.;
There are many types of detectable molecules, including coding and non-coding sequences, to meet the research needs of most customers;
Creative Bioarray has accumulated a wealth of experience, and we provide services to customers in universities, scientific research institutions and hospitals all over the world. Using our prefabricated probe products and experimental platform, we provide comprehensive FISH technical services, including experimental protocol customization and sample quality control. At the same time, we also provide full customization services for probes designed by customers. Through our advanced equipment platform and professional service team, we help customers accelerate research and development and reduce costs. You will benefit from our technical expertise, full range of products, one-stop service, and work with you to find the best solution for RNA-FISH. If you have any other requirements or questions, please contact us for cooperation. We look forward to cooperating with you in the near future.