Vero cells are the predominant host cell type used in the production of recombinant therapeutic proteins. They are chosen as hosts, because of their ability to create, fold and modify proteins in a manner that makes them compatible with the human immune system. However, Vero cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.
Karyotype analysis (G-Banded analysis) can indicate that Vero cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tools for the detection of instability in cell line development processes. Vero cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established Vero Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.
Figure 1. Vero Karyotype generated from Vero cultured cells. G-banded karyotype of Vero cells with 59 chromosomes consisted of 16 homologous pairs (blue numbers) and 13 abnormal chromosomes (black numbers).
Creative Bioarray's Vero Cell Chromosomal Aberration Analysis has the following features:
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