Vero Cell Chromosomal Aberration Analysis

Vero cells are the predominant host cell type used in the production of recombinant therapeutic proteins. They are chosen as hosts, because of their ability to create, fold and modify proteins in a manner that makes them compatible with the human immune system. However, Vero cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.

Karyotype analysis (G-Banded analysis) can indicate that Vero cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tools for the detection of instability in cell line development processes. Vero cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established Vero Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.

Figure 1. Vero Karyotype generated from Vero cultured cells. G-banded karyotype of Vero cells with 59 chromosomes consisted of 16 homologous pairs (blue numbers) and 13 abnormal chromosomes (black numbers).

The Service Features:

Creative Bioarray's Vero Cell Chromosomal Aberration Analysis has the following features:

Metaphase nuclei harvest and chromosome count.
Chromosomal abnormality quantification.
Counting: 30-40 cells, and microscope analysis of 20 metaphase spreads.
Standard cytogenetic analysis can be customized to your need.

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for considering Creative Bioarray as your Vero Cell Chromosomal Aberration Analysis Service partner.

References

Frye, C.; Deshpande, R.; Estes, S.; Francissen, K.; Joly, J.; Lubiniecki, A.; Munro, T.; Russel, R.; Wang, T.; Anderson, K. Industry view on the relative importance of “clonality” of biopharmaceutical-producing cell lines. Biologicals 2016, 44, 117–122.
Puck, T.T. The genetics of somatic mammalian cells. Adv. Biol. Med. Phys. 1957, 5, 75–101.
Gottesman, M.M. Mammalian Cell Genetics; John Wiley and Sons: New York, NY, USA, 1985.

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