Polyploid Breeding

Creative Bioarray provides probe customization services and bioinformatics services available in the field of crop genome analysis. For genome visualization problems that may be encountered in the field of ploidy hybrid breeding, our fluorescent probe customization service will assist you in probe design and tailor-made solutions for you. Our technical service platform is gradually improving the application field of analysis and testing services. Diversified products and customized solutions for customer projects are our strengths and characteristics. Choosing our FISH technical service, you will enjoy one-stop scientific research services.

Polyploids Breeding and Chromosome Analysis

Polyploid species can improve breeding efficiency by adjusting the optimal number of chromosomes to successfully match in interspecies crosses. Euploidy caused by long-range (inter-species or inter-genus) hybridization is one of the main factors in plant evolution, and is closely related to speciation and the creation of diversity. Polyploidization leads to countless genetic and epigenetic changes, ultimately resulting in species with increased genomic plasticity. Polyploidy is the result of the fusion of two or more genomes into the same nucleus, and can be classified as allopolyploid (different genome) or autopolyploid (same genome). The aneuploid chromosome addition line developed from interspecific or intergenus hybridization is an ideal choice for studying the regulation of gene expression under asymmetric genome interactions. The principle is to add a single chromosome of one species to the entire genome of another species. In addition, the difference in the ploidy level of the parent plants can lead to the occurrence of post-fertilization obstacles such as endosperm deformity and germination inhibition, which will reduce the hybridization efficiency and affect the breeding progress. Polyp. species may overcome the embryonic developmental obstacles caused by natural hybridization between species. Many crop varieties are selected in this way, such as Brassica. FISH technology can assist the genome analysis of euploid or aneuploid offspring, including karyotype analysis, chromosome drawing, parental fragment tracking, etc.

Fig 1. A case of providing FISH probes for analysis of metaphase chromosomes of male parent and allopolyploid triticale hybrids. (Evtushenko et al., 2019)

Gene Mapping and Chromosome Analysis in Ploidy Breeding

Our probe customization provides probe outsourcing services for researchers with polyploidy species research projects. In the past 10 years, in situ hybridization has been used to varying degrees in chromosomal analysis in agricultural breeding and in cytogenetic analysis. Fluorescently labeled probes provide the possibility for in situ localization of plant chromosomes or fragments in cells or tissues. In the breeding research of lily and other crops, the in situ hybridization scheme can be used for the purpose of chromosome technical analysis, chromosome variation analysis and parental chromosome tracking of hybrid offspring. Crossing of different ploidy and polyploid species and construction of aneuploid chromosome addition lines to design complex genetic events. Breeding work usually requires a long cycle, and our technical services can help customers save some time to a certain extent. If you have karyotype analysis, chromosome mapping, GISH analysis and other requirements for hybrid offspring, we can provide customized probe services for your solution.

The probe customization service provided by Creative Bioarray can be applied to gene analysis and chromosome mapping in ploidy breeding projects. Our experts can provide customers with probe design and auxiliary design services. Our high-quality custom probe products are closely related to advanced probe synthesis technology and labeling technology. You will benefit from our technical expertise and advanced equipment platform. If you need to use fluorescently labeled probes for analysis in ploidy breeding projects, you are welcome to cooperate with us. If you are also interested in our FISH technical services, please contact us for cooperation. We look forward to cooperating with you in the near future.

Reference

1. Evtushenko E V, Lipikhina Y A, Stepochkin P I, et al. Cytogenetic and molecular characteristics of rye genome in octoploid triticale (Triticosecale Wittmack)[J]. Comparative cytogenetics, 2019, 13(4): 423.