HEK293 cells are the predominant hosts for expression of recombinant proteins and are used for stable expression of proteins. However, HEK293 cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.
Karyotype analysis (G-Banded analysis) can indicate that HEK293 cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tool for the detection of instability in cell line development processes. HEK293 cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established HEK293 Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.
Figure 1. HEK293 Karyotype generated from HEK293 cultured cells. A representative G-band karyogram of a HEK-293 cell showing 71 chromosomes. Arrows indicate rearrangements, additions, and deletions.
Creative Bioarray's HEK293 Cell Chromosomal Aberration Analysis has the following features:
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