HEK293 Cell Chromosomal Aberration Analysis

HEK293 cells are the predominant hosts for expression of recombinant proteins and are used for stable expression of proteins. However, HEK293 cell line instability can affect cell culture phenotypes such as cell growth, productivity, or product quality and remain challenges for biopharmaceutical manufacturing.

Karyotype analysis (G-Banded analysis) can indicate that HEK293 cells, as well as limiting-diluted subclones, exhibit a karyotypically heterogeneous population, suggesting that chromosomal rearrangements occur spontaneously and frequently even in non-engineered host cells. This technique used to characterize the cell bank genetic instability would be evaluated as a tool for the detection of instability in cell line development processes. HEK293 cell line stability issues challenge biopharmaceutical manufacturing. Here, Creative Bioarray has established HEK293 Cell Chromosomal Aberration Analysis to fast-track your stable cell line stability and accelerate subsequent IND review.

Figure 1. HEK293 Karyotype generated from HEK293 cultured cells. A representative G-band karyogram of a HEK-293 cell showing 71 chromosomes. Arrows indicate rearrangements, additions, and deletions.

The Service Features:

Creative Bioarray's HEK293 Cell Chromosomal Aberration Analysis has the following features:

Metaphase nuclei harvest and chromosome count.
Chromosomal abnormality quantification.
Counting: 30-40 cells, and microscope analysis of 20 metaphase spreads.
Standard cytogenetic analysis can be customized to your need.

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for considering Creative Bioarray as your HEK293 Cell Chromosomal Aberration Analysis Service partner.

References

Frye, C.; Deshpande, R.; Estes, S.; Francissen, K.; Joly, J.; Lubiniecki, A.; Munro, T.; Russel, R.; Wang, T.; Anderson, K. Industry view on the relative importance of “clonality” of biopharmaceutical-producing cell lines. Biologicals 2016, 44, 117–122.
Puck, T.T. The genetics of somatic mammalian cells. Adv. Biol. Med. Phys. 1957, 5, 75–101.
Gottesman, M.M. Mammalian Cell Genetics; John Wiley and Sons: New York, NY, USA, 1985.
Binz, Regina L., et al. "Identification of novel breakpoints for locus-and region-specific translocations in 293 cells by molecular cytogenetics before and after irradiation." Scientific reports 9.1 (2019): 10554.

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