HEK293 Cell Multicolor FISH
HEK293 cell line is extensively used as a tool for mechanistic studies in mammalian biology and also for propagating viral vectors and generating
recombinant proteins. Considering its widespread use in various fields, cytogenetic characterization of HEK293 cells is critical to understanding how to
best utilize this tool for research. The HEK293 cell line exhibits a highly complex karyotype, with several numerical abnormalities and a series of
structural aberrations, including deletions and rearrangements (exchanges or translocations).
Multicolor FISH (M-FISH) assays are used for a precise assessment of complex chromosomal rearrangements. This technique uses all whole-chromosome painting
probes in multiplex-FISH and spectral karyotyping. Thus, marker chromosomes, complex chromosomal rearrangements, and all numerical aberrations can be
visualized simultaneously in a single hybridization experiment. M-FISH is a kind of versatile tool for precise chromosome separation and for rapid
chromosome classification help speeding up the workflow. Apart from that, the analysis of M-FISH images is easy even if aberrations are involved.
M-FISH complements the standard cytogenetic methods, which is helpful in deciphering HEK293 complex chromosomal rearrangements and is used to identify
non-random structural chromosome rearrangements not detectable by other methods. Creative Bioarray offers HEK293 M-FISH service for you.
Fig1. Representative
M-FISH karyotype of HEK293 cell.
Our M-FISH has unparalleled advantages:
- Rapid analysis of metaphase spread
- Ability to analyze complex cases with multiple chromosomal rearrangements, identification of marker chromosomes
- Ability to distinguish different imbalanced situations detectable in aCGH
- Ability to accurately karyotype single metaphases without selection
Features of Creative Bioarray's HEK293 Cell Multicolor FISH
- High accuracy and sensitively
- Fast turnaround time
- Competitive pricing
Quotation and ordering
Our customer service representatives are available 24hr a day! We thank you for considering Creative Bioarray as your HEK293 Cell Multicolor FISH Service
partner.
References
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Kamel, Yasser Mostafa, et al. "Fluorescence in situ hybridization assays designed for del (7q) detection uncover more complex rearrangements in
myeloid leukaemia cell lines. (2014).
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Liehr, Thomas, et al. "Multicolor FISH methods in current clinical diagnostics." Expert review of molecular diagnostics13.3 (2013): 251-255.
- Kearney, L. "Multiplex-FISH (M-FISH): technique, developments and applications." Cytogenetic and genome research 114.3-4 (2006): 189-198.
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