Creative Bioarray provides comprehensive FISH solutions for fish cytogenetic analysis, helping customers conduct research related to evolution, gene expression, and comparative genomics. A high-quality service team and excellent probe customization capabilities give us the confidence to provide customers with high-quality FISH testing services.
Fish-FISH in Cytogenetics
Fish (Pisces) exhibit the greatest biodiversity among vertebrates, offering a wide range of opportunities for cytogenetic, evolutionary, and taxonomic studies. In particular, amphibians have independently evolved many times to adapt to life on land. Since 1970, an increasing number of cytogenetic studies in fish have been carried out, mainly using conventional methods to describe karyotypes. Genomic diversity within this group has been highlighted over the past 10 years with the description of chromosomal traits such as supernumerary chromosomes, simple and multiple sex chromosome systems, polymorphisms, and polyploidy among other chromosomal features in fish. The establishment of a method for the direct preparation of chromosomes from fish tissue and the C-banding technique to detect constitutive heterochromatin on chromosomes have facilitated the study of chromosomes in fish over the years. In recent years, the study of fish chromosomes has made great progress, especially with the development of FISH technology. The method of FISH can combine the molecular information of DNA sequence with its physical location on the chromosome, thus playing an important role in the precise characterization of fish genome structure. Our FISH technology platform provides researchers with a comprehensive FISH solution for studying chromosomal differentiation, evolutionary relationships, and biodiversity.
Fig 1. Fluorescence in situ hybridization to metaphase chromosomes of distinct fish species. (Cassia F Y, et al. 2017)
FISH Solutions in Fish Cytogenetics
FISH has become an indispensable tool in fish surveys, and our platform optimizes the protocol for fish FISH in-house and gradually applies it to freshwater and marine species. These protocols can meet the diverse needs of researchers by customizing personalized probe sets. Our platform offers a variety of FISH protocols for fish cytogenetic analysis, such as many cytogenetic studies by using scattered or tandemly organized repetitive DNA sequences as probes for FISH cytogenetic mapping in different fish. In addition, we also offer the application of comparative genome hybridization (CGH) and whole chromosome painting (WCP) probes. There have been reports demonstrating the usefulness of methods for characterizing fish genomes in Zoo-FISH experiments, and our platform can provide Zoo-FISH analysis. Our FISH technology services can be used for research for different purposes, such as studying the karyotype evolution and origin and differentiation of B chromosomes and sex chromosomes. Our FISH testing service process includes isolation and preparation of probes used in fish-FISH experiments, key chromosome preparation processes, probe hybridization procedures, imaging, and data processing.
Applications
In recent years, the development and improvement of cytogenetic FISH analysis have greatly expanded the methods of chromosomal research and have played an important role in the precise characterization of fish genome structure.
- Chromosome mapping of fish and amphibians and genome-wide DNA or repeat chromosome mapping;
- Analysis of microbial infections in fish and the environment;
- for the presence of redundant chromosomes and/or mapping of repetitive sequences;
- Revealing new marine and freshwater sibling (or cryptic) species and comparisons of closely related divergent populations (ZOO-FISH);
- Comparative genomic hybridization in a suitable model of fish sex chromosome evolution;
- Chromosome mapping in cross-breeding of species of economic interest in fisheries/aquaculture;
- Assess telomere dynamics at different developmental stages for commercially produced fertile diploid individuals and sterile triploid specimens (Q-FISH);
Fig 2. Fish-FISH assay service for fish molecular cytogenetics.
If you are interested in our service, please contact us for cooperation. We look forward to cooperating with you in the near future.
Reference
- Yano, Cassia Fernanda, Luiz Antônio Carlos Bertollo, and Marcelo de Bello Cioffi. "Fish-FISH: Molecular cytogenetics in fish species." Fluorescence in situ hybridization (FISH). Springer, Berlin, Heidelberg, 2017. 429-443.
